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  • 1.
    Andersson, Helene
    et al.
    Sophiahemmet Högskola.
    Kalin, Mats
    Fossum, Bjöörn
    Sophiahemmet Högskola.
    Lindholm, Christina
    Ortqvist, Ake
    Är MRSA-patienter olika smittsamma i olika län?2009Ingår i: Läkartidningen, ISSN 0023-7205, E-ISSN 1652-7518, Vol. 106, nr 7, 412-3 s.Artikel i tidskrift (Övrigt vetenskapligt)
  • 2. Grün, Nathalie
    et al.
    Mbuya, Wilbert
    Ternhag, Anders
    Ramqvist, Torbjörn
    Ahlberg, Alexander
    Jangard, Mattias
    Sophiahemmet Högskola.
    Dalianis, Tina
    Hammarstedt-Nordenvall, Lalle
    Human papillomavirus prevalence in mouthwashes of patients undergoing tonsillectomy shows dominance of HPV69, without the corresponding finding in the tonsils.2017Ingår i: Infectious diseases (London, England), ISSN 2374-4243, Vol. 49, nr 8, 588-593 s.Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    BACKGROUND: The role of human papillomavirus (HPV) in tonsillar squamous cell carcinomas (TSCC) is of interest, since a considerable proportion of TSCC in Sweden and other Western countries is HPV positive. Nevertheless, the natural history of HPV in normal tonsils, and the progression from localized infection to pre-malignant lesion to cancer are poorly understood. The aim of this study was to investigate whether HPV types found in mouthwash samples correlated to those in tonsillar tissue from the same individuals undergoing tonsillectomy.

    METHODS: Mouthwash samples from 232 patients, aged 3-56 years, undergoing tonsillectomy, the majority with chronic tonsillitis, were collected at the time of surgery and analysed for the presence of 27 HPV types by a bead based multiplex assay.

    RESULTS: An HPV prevalence of 10.3% (24/232) was observed in mouthwash samples, with HPV 69 being the dominant type (10/24). Ten patients were positive for high risk HPV (HPV 16, 33, 35, 45, 56, 59). None of the tonsils resected from patients with HPV-positive mouthwash samples were positive for HPV.

    CONCLUSIONS: Despite an oral HPV prevalence of 10.3% in mouthwash samples from tonsillectomized patients, with dominance of HPV 69, none of the corresponding tonsillar samples exhibited the presence of HPV.

  • 3. Olofsson, Tobias C
    et al.
    Butler, Éile
    Lindholm, Christina
    Sophiahemmet Högskola.
    Nilson, Bo
    Michanek, Per
    Vásquez, Alejandra
    Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria.2016Ingår i: Current Microbiology, ISSN 0343-8651, E-ISSN 1432-0991, Vol. 73, nr 4, 463-73 s.Artikel i tidskrift (Refereegranskat)
    Abstract [en]

    In the global perspective of antibiotic resistance, it is urgent to find potent topical antibiotics for the use in human and animal infection. Healing of equine wounds, particularly in the limbs, is difficult due to hydrostatic factors and exposure to environmental contaminants, which can lead to heavy bio-burden/biofilm formation and sometimes to infection. Therefore, antibiotics are often prescribed. Recent studies have shown that honeybee-specific lactic acid bacteria (LAB), involved in honey production, and inhibit human wound pathogens. The aim of this pilot study was to investigate the effects on the healing of hard-to-heal equine wounds after treatment with these LAB symbionts viable in a heather honey formulation. For this, we included ten horses with wound duration of >1 year, investigated the wound microbiota, and treated wounds with the novel honeybee LAB formulation. We identified the microbiota using MALDI-TOF mass spectrometry and DNA sequencing. In addition, the antimicrobial properties of the honeybee LAB formulation were tested against all wound isolates in vitro. Our results indicate a diverse wound microbiota including fifty-three bacterial species that showed 90 % colonization by at least one species of Staphylococcus. Treatment with the formulation promoted wound healing in all cases already after the first application and the wounds were either completely healed (n = 3) in less than 20 days or healing was in progress. Furthermore, the honeybee LAB formulation inhibited all pathogens when tested in vitro. Consequently, this new treatment option presents as a powerful candidate for the topical treatment of hard-to-heal wounds in horses.

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