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Expression of 15-lipoxygenase type-1 in human mast cells
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2007 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, Vol. 1771, no 9, 1156-65 p.Article in journal (Refereed) Published
Abstract [en]

Mast cells play a key role in the pathophysiology of asthma. These cells exert their effector functions by releasing a variety of proinflammatory and immunoregulatory compounds. Mast cells infiltrate the bronchial epithelium and smooth muscle to a higher degree in patients with asthma compared to control subjects. 15-Lipoxygenase type-1 (15-LO-1) is a prooxidant enzyme which is expressed in asthmatic lungs leading to formation of pro- and anti-inflammatory mediators. Here we report that interleukin-4 (IL-4) induced the expression of 15-LO-1 in human cord blood derived mast cells (CBMC) as demonstrated by RT-PCR, western blot and immunocytochemistry. The major metabolite of arachidonic acid formed via the 15-LO pathway in IL-4 treated CBMC was identified as 15-ketoeicosatetraenoic acid (15-KETE, also named 15-oxo-ETE) with smaller amounts of 15-hydroxyeicosatetraenoic acid (15-HETE) as identified by HPLC and mass spectrometry (MS/MS). Furthermore, immunohistochemical stainings demonstrated the expression of 15-LO-1 in mast cells in lung and skin in vivo. Osmotic activation of CBMC with mannitol resulted in activation of the 15-LO-1 pathway. In conclusion, the expression of 15-LO-1 and release of 15-LO-1 derived products by mast cells may contribute to the role of these cells in asthma and other inflammatory diseases.

Place, publisher, year, edition, pages
2007. Vol. 1771, no 9, 1156-65 p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:shh:diva-54DOI: 10.1016/j.bbalip.2007.06.001PubMedID: 17662651OAI: oai:DiVA.org:shh-54DiVA: diva2:301952
Available from: 2010-03-03 Created: 2010-02-23 Last updated: 2014-10-15Bibliographically approved

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Kumlin, Maria
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CiteExportLink to record
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Citation style
  • apa
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  • modern-language-association-8th-edition
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  • de-DE
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