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Release of prostaglandin D2 and leukotriene C4 in response to hyperosmolar stimulation of mast cells
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2006 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, E-ISSN 1398-9995, Vol. 61, no 12, 1473-9 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND: Mannitol-induced bronchoconstriction in subjects with exercise-induced asthma is associated with increased urinary excretion of 9alpha, 11beta-PGF(2), a metabolite of prostaglandin D(2) (PGD(2)) serving as a mast cell marker. It has however been questioned whether or not human mast cells release PGD(2) and leukotriene C(4) (LTC(4)) after osmotic challenge with mannitol in vitro. METHODS: Cord blood-derived human mast cells were stimulated osmotically, immunologically or with a combination of both. Supernatants were analysed for PGD(2), LTC(4) and histamine contents with enzyme immunoassays. RESULTS: Significant release of de novo synthesized eicosanoids, predominantly PGD(2) [12 (8.8, 14) pmol/10(6)cells; median (25th, 75th percentile) but also LTC(4) (0.1 (0.08, 0.15) pmol/10(6) cells] were found in mast cells in vitro in response to 0.7 M mannitol stimulation. A massive release of histamine [70 (5.3)% of total; mean (SEM)] was also found. There were no correlations between the levels of released mediators after mannitol stimulation. In contrast, there was a correlation between release of PGD(2) and LTC(4), following immunological stimulation. CONCLUSION: The findings support that hyperosmolar challenge activates mast cells, but different than antigen stimulation.

Place, publisher, year, edition, pages
2006. Vol. 61, no 12, 1473-9 p.
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Medical and Health Sciences
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URN: urn:nbn:se:shh:diva-56DOI: 10.1111/j.1398-9995.2006.01213.xPubMedID: 17073880OAI: oai:DiVA.org:shh-56DiVA: diva2:301323
Available from: 2010-03-03 Created: 2010-02-23 Last updated: 2014-10-15Bibliographically approved

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Kumlin, Maria
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CiteExportLink to record
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